Syngeneic anti-idiotypic antibody prevents localization of a murine monoclonal antibody in human tumour xenografts
โ Scribed by Pimm, Malcolm V.; Baldwin, Robert W.
- Book ID
- 121929557
- Publisher
- Elsevier Science
- Year
- 1990
- Weight
- 315 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0277-5379
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A syngeneic anti-idiotype monoclonal antibody (MAb) (CM-I I) directed against an anti-carcinoembryonic antigen (CEA) murine MAb (NP-4) was evaluated as a second antibody (SA) to promote the rapid clearance of radiolabeled NP-4 from the blood. Initial studies confirmed that CM-I I IgG removed l3Il-NP
BALB/c mice were immunized against syngeneic murine 79 I T136 monoclonal antibody (MAb) by intraperitoneal (i.p.) injection of the antibody conjugated to ricin toxin A chain. Subsequently, in these and control mice. the biodistribution of radioiodinated 79 I T/36 antibody and isotype-matched (IgGlb)
To demonstrate the precise distribution and binding of in vivo injected monoclonal antibodies on histological tumour sections, we have biotinylated our primary antibody AUA I. Biotinylated antibody was injected into nude mice bearing simultaneous subcutaneous and intraperitoneal xenografts of the hu