Synergistic effect of interleukin-1β and tumor necrosis factor α on interleukin-8 gene expression in synovial fibroblasts. evidence that interleukin-8 is the major neutrophil-activating chemokine released in response to monokine activation
✍ Scribed by Palaniswami Rathanaswami; Mohamed Hachicha; Wai Lee Wong; Thomas J. Schall; Shaun R. Mccoll
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 953 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
✦ Synopsis
To investigate both the involvement of chemokines in general and the relative importance of specific chemokines in rheumatoid arthritis (RA), we characterized the effect of the monokines tumor necrosis factor a (TNFa) and interleukin-lp (IL-1p) on the synthesis of neutrophil-activating factors by synovial fibroblasts isolated from the joints of patients with RA.
Methods. Neutrophil-stimulating activity was assessed by determining intracellular calcium mobilization. IL-8 synthesis and secretion was assessed by specific enzyme-linked immunosorbent assay, and IL-8 messenger RNA (mRNA) levels were determined by Northern blot.
Results. Treatment of synovial fibroblasts with IL-1p and TNFa resulted in the production of an activity which induced intracellular calcium mobilization in peripheral blood neutrophils. The 2 monokines From the