Synchrotron Radiation and Structural Proteomics

✍ Scribed by Eugenia Pechkova, Christian Riekel

• Publisher: Pan Stanford Publishing
• Year: 2011
• Tongue: English
• Leaves: 514
• Series: Pan Stanford Series on Nanobiotechnology
• Edition: 1
• Category: Library

✦ Synopsis

This book presents an overview of the current state of research in both synchrotron radiation and structural proteomics from different laboratories worldwide. The book presents recent research results in the most advanced methods of synchrotron radiation analysis, protein micro- and nano crystallography, X-ray scattering and X-ray optics, coherent X-Ray diffraction, and laser cutting and contactless sample manipulation are described in details. The book focuses on biological applications and highlights important aspects such as radiation damage and molecular modeling.

✦ Table of Contents

Dedication......Page 6
Contents......Page 8
Preface......Page 16
Section I: Source and Instrumentation......Page 18
1. Protein Micro- and Nanocrystallography Using Synchrotron Radiation......Page 20
2. Single-Bounce Monocapillary X-Ray Optics: Design and Biological Applications......Page 48
3. High Field Superconducting Magnets for Generation of Synchrotron Radiation......Page 76
Section II: Methods......Page 120
4. Coherent X-Ray Diffraction for High-Contrast Bioimaging......Page 122
5. Model Lysozyme Crystal Versus Aggregate (Un)Confined Formation as Viewed by Recent Theory Aided by Computer Experiment......Page 142
6. Optical Tweezers for Touchless Sample Manipulation in Synchrotron Radiation Experiments......Page 200
7. Molecular Modeling to Facilitate Protein Crystallization......Page 230
8. Gold Nanoparticle Thin Films on Glass: Influence of Film Thickness and Annealing Time......Page 262
Section III: Structural Proteomics......Page 280
9. Atomic Structure and Radiation Resistance of Langmuir–Blodgett Protein Crystals......Page 282
10. Toward the Understanding of Molecular Aspects of Helicobacter Pylori cag-PAI......Page 312
11. Thermophilic Enzymes of Potential Industrial Use: Structure and Function......Page 336
12. Using X-ray Scattering to Study the Structures of Membrane-Associated proteins......Page 374
13. Structural Analysis of the β-Subunit of the Translation Initiation Factor alF2 from Different Species: Role of Zn Ions......Page 390
14. Crystal Quality: A Quest for Structural Proteomics......Page 404
15. Growth and Organization of Langmuir–Blodgett Protein Crystals via In Situ GISAXS, Laser-Microdissection, Nanodiffraction, Raman Spectroscopy, and Atomic Force Microscopy......Page 430

✦ Subjects

Биологические дисциплины;Молекулярная биология;