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Synchronization of Mammalian Cells with Tritiated Thymidine

โœ Scribed by Whitmore, G. F.; Gulyas, S.

Book ID
121175806
Publisher
American Association for the Advancement of Science
Year
1966
Tongue
English
Weight
494 KB
Volume
151
Category
Article
ISSN
0036-8075

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Exponentially growing L-cells were synchronized by the double thymidine-block method and exposed to high specific activities of tritiated thymidine. DNA, RNA, and protein synthetic rates were measured through one cell cycle with 1-hour pulses of the appropriate Ci4-labelled precursors. Equivalent do