Synchronised transmembrane insertion and glycosylation of a nascent membrane protein
โ Scribed by Rothman, James E.; Lodish, Harvey F.
- Book ID
- 109707488
- Publisher
- Nature Publishing Group
- Year
- 1977
- Tongue
- English
- Weight
- 879 KB
- Volume
- 269
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/269775a0
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The inner membrane protein YidC is associated with the preprotein translocase of Escherichia coli and contacts transmembrane segments of nascent inner membrane proteins during membrane insertion. YidC was purified to homogeneity and co-reconstituted with the SecYEG complex. YidC had no effect on the
## Abstract A membrane protein with two transmembrane domains was synthesized by means of the thioester method. The F~1~F~0~ ATP synthase subunit c (Sub.c), which consists of 79 amino acid residues (MW 8257), was chosen as a target. For synthetic purposes, two building blocks, Bocโ[Lys^34^(Boc)]โSu