Sympathetic nerve contact causes maturation of mast cellsin vitro

✍ Scribed by Blennerhassett, Michael G. ;Bienenstock, John

• Book ID: 101259213
• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 320 KB
• Volume: 35
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199805)35:2<173::aid-neu4>3.0.co;2-1

✦ Synopsis

Using a tissue culture model devel-RBL cells cocultured with neurons for 24-48 h took oped to study interactions between peripheral neurons up significantly more 3 H-5HT and released a signifiand mast cells (MC), time-lapse microscopy showed cantly larger percentage of 3 H-5HT in response to the that RBL-2H3 cells (a model of the mucosal MC) calcium ionophore A23187 than RBL cells in parallel formed attachments with sympathetic neurons, ceased pure cultures. Since no change in MC phenotype was to divide, and moved along neurites toward the cell seen, we conclude that contact with nerve membrane bodies. Electron microscopy showed significant inmay be a developmental cue leading to maturation of crease in granules compared to intrinsic controls MC.