SYBR green and TaqMan real-time PCR assays are equivalent for the diagnosis of dengue virus type 3 infections
✍ Scribed by Alessandra Cristina Gomes-Ruiz; Renata Teodoro Nascimento; Sérgio Oliveira de Paula; Benedito Antônio Lopes da Fonseca
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 87 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0146-6615
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✦ Synopsis
Abstract
Dengue is the most important arthropod‐borne viral disease in the world. A rapid diagnostic test for dengue is warranted, and real‐time polymerase chain reaction may improve diagnosis. TaqMan and Sybr Green systems were evaluated for the diagnosis of dengue virus type 3 (DENV‐3) infections. Out of 77 patients with clinical suspicion of dengue infection, specific IgM antibodies were detected in 40 patients. DENV‐3 was detected and quantitated in 17 IgM‐positive samples by both systems. These assays were shown to be rapid, and specific for detection of DENV‐3, and that for diagnostic purposes, there is no difference between these two assays. J. Med. Virol. 78:760–763, 2006. © 2006 Wiley‐Liss, Inc.