Switch of substrate specificity of hyperthermophilic acylaminoacyl peptidase by combination of protein and solvent engineering
✍ Scribed by Chang Liu; Guangyu Yang; Lie Wu; Guohe Tian; Zuoming Zhang; Yan Feng
- Book ID
- 107692115
- Publisher
- SP Higher Education Press
- Year
- 2011
- Tongue
- English
- Weight
- 572 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1674-800X
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## Abstract An __Escherichia coli__ suppressor tRNA^Phe^ (tRNA^Phe^~CUA~) was misacylated with 4‐iodo‐L‐phenylalanine by using the A294G phenylalanyl–tRNA synthetase mutant (G294‐PheRS) from __E. coli__ at a high magnesium‐ion concentration. The preacylated tRNA was added to an __E. coli__ cell‐fre