Survival oflux-marked bacteria introduced into soil and the rhizosphere of bean (Phaseolus vulgarisL.)
✍ Scribed by J. Kozdrój
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 554 KB
- Volume
- 12
- Category
- Article
- ISSN
- 1573-0972
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✦ Synopsis
The survival of lux-marked recombinants of Escherichia coli and Bacillus subtilis was studied in the rhizosphere of bean (Phaseolus vulgaris L.) and in bulk soil. The number of E. coli (pSB343) containing a complete lux operon did not differ significantly according to whether they were introduced into soil separately or together with a non-luminescent mutant Pseudomonas fluorescens R2fN. When genetically altered strains of E. coli and B. subtilis bearing a complete or an incomplete lux-reporter system were introduced into soil, the numbers of surviving cells were the same both in the rhizosphere and bulk soil. The insertion of lux genes into bacterial strains therefore does not affect their competitiveness and survival in the rhizosphere and bulk soil.