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Survival of mouse bone-marrow cells frozen and thawed in solutions of amino acids

โœ Scribed by T.T. Phan; M.A. Bender

Book ID
118874042
Publisher
Elsevier Science
Year
1960
Tongue
English
Weight
245 KB
Volume
20
Category
Article
ISSN
0014-4827

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Eight-cell mouse embryos were frozen by using glycerol, ethylene glycol, or erythritol as the cryoprotectant. The samples were slowly cooled (0.5"C/min) to temperatures between -10 and -79ยฐC before direct transfer to liquid nitrogen (-196ยฐC) and thawed slowly ( -20"C/min) or rapidly ( -500ยฐC/min). T