Survival and toxicity of xenogeneic murine retroviral vector producer cells in liver

✍ Scribed by Donald W. Moorman; Daniel A. Butler; J. Daniel Stanley; Jesse L. Lamsam; Kenneth W. Culver; Mark R. Ackermann; Carol D. Jacobson

• Book ID: 102926401
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 581 KB
• Volume: 57
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/jso.2930570304

✦ Synopsis

Murine retroviral vector producer cells (VPC) can selectively transfer genes stably into proliferating cells. We injected LacZ gene producing VPC directly into nonnal rat liver. There was no measurable gene transfer into the surrounding hepatic parenchyma with X-GAL staining. Rejection of the xenogeneic murine VPC occurred 7-14 days after injection. Toxicity of this delivery method was evaluated with the herpes sirnplex-thymidine kinase (HS-tk) gene, which confers sensitivity to the antiherpes drug, ganciclovir (GCV). HS-tk VPC were injected and allowed to grow in normal liver for 7 days before GCV treatment. There was no clinical or histologic evidence of toxicity with GCV treatment. These findings suggest that the direct injection of murine VPC into xenogeneic human tumors may survive sufficiently long without immunosuppression to transfer genes to tumor cells in situ without attendant toxicity.