Surface-modified TiO2 nanoparticles as affinity probes and as matrices for the rapid analysis of phosphopeptides and proteins in MALDI-TOF-MS

✍ Scribed by Yaotang Ke; Suresh Kumar Kailasa; Hui-Fen Wu; Mohd Nawaz

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 448 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.201000415

✦ Synopsis

Surface-modified TiO 2 nanoparticles as affinity probes and as matrices for the rapid analysis of phosphopeptides and proteins in MALDI-TOF-MS

We utilized three different types of TiO 2 nanoparticles (NPs) namely TiO 2 -dopamine, TiO 2 -CdS and bare TiO 2 NPs as multifunctional nanoprobes for the rapid enrichment of phosphopeptides from tryptic digests of aand b-casein, milk and egg white using a simplified procedure in MALDI-TOF-MS. Surface-modified TiO 2 NPs serve as effective matrices for the analysis of peptides (gramicidin D, HW6, leucine-enkephalin and methionine-enkephalin) and proteins (cytochrome c and myoglobin) in MALDI-TOF-MS. In the surface-modified TiO 2 NPs-based MALDI mass spectra of these analytes (phosphopetides, peptides and proteins), we found that TiO 2 -dopamine and bare TiO 2 NPs provided an efficient platform for the selective and rapid enrichment of phosphopeptides and TiO 2 -CdS NPs efficiently acted as the matrix for background-free detection of peptides and proteins with improved resolution in MALDI-MS. We found that the upper detectable mass range is 17 000 Da using TiO 2 -CdS NPs as the matrix. The approach is simple and straightforward for the rapid analysis of phosphopeptides, peptides and proteins by MALDI-MS in proteome research.