Surface modification of polymers. V. Biomaterial applications

✍ Scribed by Klas Allmér; J. Hilborn; Per H. Larsson; A. Hult; B. Rånby

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 560 KB
• Volume: 28
• Category: Article
• ISSN: 0887-624X
• DOI: 10.1002/pola.1990.080280112

✦ Synopsis

Abstract

Polyethylene films were surface grafted with glycidyl methacrylate (GMA) by UV irradiating the film for 5 min together with benzophenone. Poly(ethylene glycol) (PEG) was attached to the grafted surface through reaction with the epoxy groups. This yielded a surface which consisted of 95% PEG as measured with ESCA. The adsorption of human transferrin onto this film was significantly reduced as compared with a pure polyethylene film. Heparin was also reacted with a GMA grafted PE surface. ESCA showed that heparin was grafted to the surface, and in vitro blood clotting tests on the heparinized PE surface showed a reduced thrombus formation. GMA grafted polystyrene wells were reacted with carbohydrazide, to the formed carbohydrazide surface a rabbit antibody raised against mouse urinary protein (RaMUP) was covalently coupled. The RaMUP coupled surfaces was used in the detection of mouse urinary protein (MUP) at low concentrations (ca. 1 ng/mL) with an ELISA technique.