Surface modification of poly(aryl ether ether ketone) (PEEK) film by covalent coupling of amines and amino acids through a spacer arm
✍ Scribed by Olivier Noiset; Yves-Jacques Schneider; Jacqueline Marchand–Brynaert
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 251 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0887-624X
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✦ Synopsis
Using the wet-chemistry technique, we selectively reduced the surface of the PEEK film (PEEK-OH), and covalently fixed hexamethylene diisocyanate by addition onto the hydroxyl functions. The resulting PEEK-NCO film displayed free isocyanate termini, the basic hydrolysis of which gave the PEEK-NH 2 film in 85% extent of derivatization. The PEEK-NCO film reacted with trifluoroethylamine in toluene, trifluoroethylamine in PBS buffer, GABA, and lysine in PBS buffer to furnish, respectively, the PEEK-CF 3 (A), PEEK-CF 3 (B), PEEK-CO 2 H, and PEEK(NH 2 )CO 2 H films in 80%, 45%, 30%, and 25% extents of derivatization, as determined from the F/C and N/C atomic ratios recorded in the corresponding XPS spectra. The surface reactivity of the PEEK-NH 2 and PEEK-NCO films was assayed by coupling with appropriate 3 H labels followed by liquid scintillation counting of the sample-associated radioactivity. The PEEK-NH 2 , PEEK-CO 2 H, and PEEK-(NH 2 )CO 2 H films were used as substrates for the cultivation of CaCo 2 epithelial cells; the presence of surface amine and carboxyl functions significantly improves the cellular adhesion and growth.