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Surface modification of medical-grade polyurethane by cyanurchloride-activated tetraether lipid (a new approach for bacterial antiadhesion)

✍ Scribed by Ananthu Sateesh; Jürgen Vogel; Evelyn Dayss; Beate Fricke; Karin Dölling; Ulrich Rothe


Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
457 KB
Volume
84A
Category
Article
ISSN
1549-3296

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✦ Synopsis


Abstract

The surface of high‐grade medical polyurethane was chemically modified and coated with tetraether lipid, employing cyanurchloride as coupling agent. The surfaces were initially grafted with hexamethylene diisocyanate and subsequently treated with water or hexamethylene diamine to generate free amino groups on the surface. This provides a convenient way for covalent coupling of tetraether lipids. These lipid‐coated surfaces were investigated for bacterial adhesion using Pseudomonas aeruginosa. All lipid‐coated surfaces significantly reduced bacterial adhesion. Surface topology and individual modification steps were controlled by contact angle measurements, attenuated total reflectance infrared spectroscopy, X‐ray photoelectron spectroscopy, confocal laser scanning microscopy, and environmental scanning electron microscopy. This new surface modification approach may reduce the adhesion of bacteria on biomaterials. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008