Surface membrane electrokinetic properties of polymorphonuclear leucocytes: Subpopulation heterogeneity and phagocytic competence
✍ Scribed by P. Spangenberg; N. Crawford
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 587 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
In cells which display plasma membrane-mediated motile activities (locomotion, pseudopodia formation, chemotaxis, phagocytosis, etc.) a variety of intrinsic plasma membrane constituents, some of which contribute to the cell's electrokinetic properties, have been indicted as participants in the motile phenomena. In particular, some attention has been focused upon certain membrane sialoglycoproteins as possible candidates for transmembrane control of the disposition and level of organisation of cytoskeletal elements such as actin, actin binding protein, and a-actinin. In some way, not yet understood, these structural and contractile proteins interact to produce force generation for the motile event.
A relationship between surface membrane electrical charge and motile phenomena has been speculated upon by a number of researchers. It appears that in many motile cells there is an inverse relationship between surface membrane electronegativity (zeta potential) and functional competence. For example, as early as 1966, Weiss and his colleagues showed that removal of sialic acid groups from the surfaces of monocytes and macrophages by neuraminidase resulted in enhanced phagocytic activity. In a similar study, Lichtman and Weed showed that mature granulocytes from marrow, characterised by a high neuraminidase-labile negative surface charge density,