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Surface markers of human lymphokine-activated killer cells and their precursors. Analysis at the population and clonal level

✍ Scribed by Silvano Ferrini; Lorenzo Moretta; Giuseppe Pantaleo; Alessandro Moretta

Publisher
John Wiley and Sons
Year
1987
Tongue
French
Weight
700 KB
Volume
39
Category
Article
ISSN
0020-7136

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✦ Synopsis

Lymphokine

-activated killer (LAK) activity was first analyzed on PBL populations fractionated on the basis of the expression of TI1 or T3 antigen. LAK cell precursors were found to be present in both T I I + and TI Ipopulations, but only in the T3cell fraction. The generation of LAK activity in highly purified T3populations of PBL was not accompanied by expression of T3 antigen during a 5-day culture period. LAK activity was next analyzed at the level of limiting dilution clonal microcultures. TI I +T3and TI I +T3+ cells, cloned under optimal culture conditions, gave a frequency of proliferating cells of approximately I cell in 1.25 for TI I + T3 + and I cell in 10 for TI1 +T3cells. Clones were screened for their ability to lyse fresh ovarian carcinoma cells and K562 target cells. The majority of LAK clones were derived from the Tll+T3cells; moreover, most of the clones derived from these cells displayed LAK activity. Clones displaying LAK activity lysed a panel of fresh or cultured tumor target cells, but failed to lyse PHA-activated lymphoblasts. Surface marker analysis indicated that all the clones had maintained the original TI I n 3 phenotype. Whereas 2 T3+ selected LAK clones expressed the T8+T4phenotype, only I out of 9 T3clones was T8+T4-, all the others lacking both T4 and T8 antigens.

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