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Surface glycoproteins of differentiating neuroblastoma cells analyzed by lectin binding and flow cytometry

✍ Scribed by Mary F. D. Notter; James F. Leary

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
738 KB
Volume
8
Category
Article
ISSN
0196-4763

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✦ Synopsis

Cell surface glycoproteins of mitotic neuroblastoma cells and cells differentiated by prostaglandin cyclic adenosine monophosphate treatment were quantified by flow cytometric analysis and specific fluorescent lectins. No differences in fluorescent lectin binding were seen between suspensions of mitotically active and differentiated NzAB-1 cells following exposure to either fluorescein (FL)-labeled soy bean agglutinin (FL-SBA) specific for N acetyl galactosamine or FLconcanavalin A (FL-CON A

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## Background: The primary source of interference in immunofluorescence measurements by flow cytometry is background autofluorescence. Methods: Using human lung fibroblasts (HLFs) as an autofluorescent cell model, unfixed HLFs and HLFs fixed in methanol, ethanol, formaldehyde, paraformaldehyde and