Surface-enhanced resonance Raman spectroscopy of iron-dopamine complexes

✍ Scribed by Will K Kowalchyk; Kevin L Davis; Michael D Morris

• Book ID: 103908039
• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 488 KB
• Volume: 51
• Category: Article
• ISSN: 1386-1425
• DOI: 10.1016/0584-8539(94)00153-3

✦ Synopsis

Surface-enhanced resonance Raman spectroscopy (SERRS) at silver colloids is used to detect the catecholamines, 3-hydroxytyramine (dopamine) and 3,4-dihydroxyphenylacetic acid (DOPAC), in a modified Ringer's solution. Catecholamines form very strong complexes with iron(III) in solution (K& 104~') and exhibit a broad ligand-to-metal charge-transfer (LMCT) absorption in the visible (-500 nm). Resonance enhancement is achieved by excitation at 532 nm from a frequency doubled Nd :YAG laser with high quality spectra attainable in 1 s. Maximum SERRS signal is observed when basic buffer is added to a dopamine sample containing 50× 10 6M ferric ion. Dopamine concentrations in the nanomolar (resting level) range are obtained using this technique.