Surface-enhanced Raman scattering and fluorescence spectroscopy reveal molecular interactions of all-trans retinoic acid and RARγ ligand-binding domain
✍ Scribed by H. Morjani; A. Beljebbar; G. D. Sockalingum; T. A. Mattioli; D. Bonnier; H. Gronemeyer; M. Manfait
- Book ID
- 101293546
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 171 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1075-4261
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✦ Synopsis
Surface-enhanced Raman scattering and fluorescence were used to investigate the interactions of all-trans retinoic acid with the gamma-type retinoic acid receptor. Raman data revealed a significant attenuation in intensity of the bands originating from the retinoic acid polyenic chain upon receptor binding, with the spectrum being dominantly that of the b-ionone ring. Fluorescence measurements supported the hydrophobic character of the ligand binding. These novel spectroscopic results are fully consistent with the published X-ray crystallographic data and suggest that these techniques may be valuable additional tools to characterize the interactions of agonists and antagonists with residues in the ligand-binding pockets of retinoid receptor homo-and heterodimers.