Surface-enhanced Raman assays (SERA): measurement of bilirubin and salicylate

✍ Scribed by Roberta Sulk; Collin Chan; Jason Guicheteau; Cieline Gomez; J. B. B. Heyns; Robert Corcoran; Keith Carron

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 156 KB
• Volume: 30
• Category: Article
• ISSN: 0377-0486
• DOI: 10.1002/(sici)1097-4555(199909)30:9<853::aid-jrs457>3.0.co;2-s

✦ Synopsis

Results of a study to detect bilirubin and salicylate using SERS and a reactive coating are presented. The motivation for this work was to develop an assay that would simplify an analysis by detecting the analyte in a whole blood sample. This would remove the risks and time associated with preparing a serum sample. Quantitative analysis was performed using partial least-squares. The results are that one can detect bilirubin and salicylate in whole blood at levels below the normal or therapeutic levels using a coating based on an argentiphilic diazonium molecule. The sharp Raman bands associated with the analyte-coating adduct are sufficiently resolved to allow the analyte to be detected in the presence of a complex matrix such as whole blood.