𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Surface-Density and Orientation Effects on Immobilized Antibodies and Antibody Fragments

✍ Scribed by Spitznagel, Thomas M. ;Clark, Douglas S.

Book ID
109905607
Publisher
Nature Publishing Group
Year
1993
Tongue
English
Weight
906 KB
Volume
11
Category
Article
ISSN
1087-0156

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There are numerous chemical methods published that enable protein coupling to carboxymethyl (CM) dextran. Here we have taken traditional amine coupling using N-hydroxysuccinimide (NHS) and N'-(3dimethylaminopropyl) carbodiimide hydrochloride (EDC) and coupled an antibody fragment (scFv) to CM dextra