Surface Coating with a Thermoresponsive Copolymer for the Culture and Non-Enzymatic Recovery of Mouse Embryonic Stem Cells

✍ Scribed by Xian Jun Loh; Jiansheng Gong; Makoto Sakuragi; Takashi Kitajima; Mingzhe Liu; Jun Li; Yoshihiro Ito

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 657 KB
• Volume: 9
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.200900081

✦ Synopsis

Abstract

A thermoresponsive substrate based on a triblock copolymer, poly(N‐isopropylacrylamide)‐block‐poly[(R)‐3‐hydroxybutyrate]‐block‐poly(N‐isopropylacrylamide) (PNIPAAm‐PHB‐PNIPAAm), co‐coated with gelatin, was developed for the culture and non‐enzymatic recovery of mouse embryonic stem cells. After culture, the cells could be detached by cooling at 4 °C for 20 min without trypsin digestion. The embryonic stem cells remained undifferentiated after culture on the gelatin/copolymer‐coated surfaces, similar to standard culture techniques. Overall, the triblock copolymer coating was superior to the PNIPAAm homopolymer coating in terms of supporting better cell growth, being more stable, presenting a more homogeneous surface coating, and maintaining pluripotency of the embryonic stem cells.

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