Surface charge and cell volume of synchronized mouse lymphoblasts (L5178-Y)

✍ Scribed by Brenda Buckhold Shank; H. John Burki

• Book ID: 102881134
• Publisher: John Wiley and Sons
• Year: 1971
• Tongue: English
• Weight: 576 KB
• Volume: 78
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040780211

✦ Synopsis

Cellular electrophoretic mobility, and therefore, surface charge/ unit area, was found to be constant throughout the cell cycle of synchronized L5178-Y mouse lymphoblasts, remaining at -1.21 p sec-' V-l cm. Measured cell volumes of these synchronized cells increased linearly over the cell cycle and at mitosis each cell divided into two cells, each cell having half of the parent ~0 1ume. An hypothesis is presented which explains the presence of a mobility peak at mitosis in cells which normally are grown attaching to a glass surface on the basis of differential morphological changes during mitosis; cells such as the L5178-Ys, which do not attach to glass normally, do not undergo such morphological changes at mitosis and, therefore, would not demonstrate a mobility peak at that time.