A rectangular electrophoresis chamber has been constructed cal electrophoresis chamber whose surface served as the whereby electroosmotic fluid mobility can be determined at small experimental substrate. Unfortunately, with the cylindrical nontransparent sample plates. A hydrodynamic description of capillary electrophoresis chamber configuration, choice of fluid flow in the chamber is forwarded, and verified, for accurate experimental substrate was limited to transparent materials determination of particle mobility and electroosmosis at sample that could be drawn into a capillary and retain the optical
plates. Advantages of the chamber design and technique include
properties necessary to view the reference particle. In the the ability to determine electroosmotic fluid mobility at sample interest of extending the range of surfaces which could be plates from a single particle mobility measurement within the characterized electrokinetically, there was a need to measure chamber. As demonstration of the usefulness of the technique, characterization of several polymer surfaces deposited as a thin electroosmosis at nontransparent flat plates and a desire that film from a cold gas plasma was performed by determining the the characterization be complemented with other techniques, pH dependence of electroosmotic fluid flow. The plasma polymer e.g., ellipsometry, ESCA, and contact angle. Thus, a more surfaces include those of plasma polymerized 1,2-diaminocyclocomplete description of factors influencing surface phenomhexane, acrylic acid, and hexamethyldisiloxane. Results of the ena such as protein adsorption could be attained. characterization include quantification of ionizable surface groups Electroosmosis has been measured at flat plates in the with concomitant determination of p K values of these groups. The past (3-5). Such measurements were made in a rectangular values obtained have proven useful to explain trends in protein microelectrophoresis cell where sample plates serve as the adsorption at these surfaces.