Surface area ratios II. A stereological method for estimating changes in average cell volume and frequency

✍ Scribed by Bolender, Robert P.

• Publisher: John Wiley and Sons
• Year: 1979
• Tongue: English
• Weight: 594 KB
• Volume: 195
• Category: Article
• ISSN: 0003-276X
• DOI: 10.1002/ar.1091950202

✦ Synopsis

Abstract

The study describes a stereological method for detecting relative changes in cells‐with respect to their average volumes and to their frequencies within one cm^3^ of exocrine cells. The method is based on surface area ratios (Bolender, '1979) and can detect changes comparable to those obtained with the numerical density approaches (Loud, '68; Weibel et al., '69; Bolender, '74; Williams, '77). The new method requires only intersection counts‐ the same ones that are used for surface density estimates‐ and, as a result, avoids the difficult and often problematic procedures of the numerical densities. In analyzing the cellular changes in vitro, the choice of either a zero or timed control was found to exert a major influence on the results. Timed controls were required to “isolate” the experimentally induced changes from those Produced by the incubation medium.