Suppressive effects of extracts from the aerial part of Coriandrum sativum L. on LPS-induced inflammatory responses in murine RAW 264.7 macrophages
✍ Scribed by Trang-Tiau Wu; Chia-Wen Tsai; Hsien-Tsung Yao; Chong-Kuei Lii; Haw-Wen Chen; Yu-Ling Wu; Pei-Yin Chen; Kai-Li Liu
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 365 KB
- Volume
- 90
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Background:
Coriandrum sativum is used not only as a spice to aid flavour and taste values in food, but also as a folk medicine in many countries. since little is known about the anti-inflammatory ability of the aerial parts (stem and leaf) of c. sativum, the present study investigated the effect of aerial parts of c. sativum on lipopolysaccharide (lps)-stimulated raw 264.7 macrophages. we further explored the molecular mechanism underlying these pharmacological properties of c. sativum.
Results:
Ethanolic extracts from both stem and leaf of c. sativum (csee) significantly decreased lps-induced nitric oxide and prostaglandin e(2) production as well as inducible nitric oxide synthase, cyclooxygenase-2, and pro-interleukin-1beta expression. moreover, lps-induced ikappab-alpha phosphorylation and nuclear p65 protein expression as well as nuclear factor-kappab (nf-kappab) nuclear protein-dna binding affinity and reporter gene activity were dramatically inhibited by aerial parts of csee. exogenous addition of csee stem and leaf significantly reduced lps-induced expression of phosphorylated mitogen-activated protein kinases (mapks).
Conclusion:
Our data demonstrated that aerial parts of csee have a strong anti-inflammatory property which inhibits pro-inflammatory mediator expression by suppressing nf-kappab activation and mapk signal transduction pathway in lps-induced macrophages.