Suppression of UV- and interferon-α-refractoriness by antipain in human IFr cells established from RSa cells sensitive to both stimuli
✍ Scribed by Nobuo Suzuki
- Book ID
- 102654905
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 539 KB
- Volume
- 167
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
The human cell line IF' is a variant with an increased resistance to cell proliferation inhibition (CPI) by human interferon (HuIFN)-a, established from RSa cells with unusually high-sensitivity to CPI. IF' cells were later found to have increased resistance to the cell-killing effects of far-ultraviolet (UV) irradiation. Here, in cell lysates extracted from UV-irradiated IF' cells but not in those from irradiated RSa cells, fibrinolytic protease activity was found to be elevated promptly and transiently after irradiation. Treatment of IF' cells with HulFN-a alone also resulted in the elevation of protease activity, but not that of RSa cells. Both the activity elevated after UV irradiation and after HulFN-a treatment was inhibited to the greatest extent by antipain in vitro. Moreover, the refractoriness of IF' cells to UV cell-killing and to HulFN-a CPI was suppressed by culturing with medium containing antipain immediately after UV irradiation or during HulFN-a exposure. In similarly treated RSa cells, there was no modulation of UV-or HulFN-asusceptibility. These comparative characteristics between the two cell lines sug- gested that antipain-sensitive proteases and/or cellular functions may be involved in increased resistance to UV and HulFN-a of IF' cells. o 1996 Wiley-Liss, Inc.
Cell proliferation inhibition (CPI) is one of the pleiotropic effects of human intederon (Hu1FN)-a, but much remains unknown concerning the molecular mechanisms underlying the CPI effect (Pestka, 1992;Sen and Lengyel, 1992;Pellegrini and Schindler, 1993). To investigate the mechanisms of CPI, a pair of human cell lines differing in their sensitivity to CPI may be powerful tools; RSa and IF' cells satisfy these criteria, respec-