Suppression of tumorigenicity, but not invasion, in glioblastoma/HeLa cell hybrids

✍ Scribed by Kevin Ess; Haiyan Chen; Ann Kier; Robert Brackenbury

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 889 KB
• Volume: 162
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041620306

✦ Synopsis

Abstract

Somatic cell hybrids between SNB‐19 human glioblastoma cells and human D98^OR^ HeLa parental cells were produced and analyzed for their ability to form tumors in nude mice and to invade reconstituted extracellular matrix (Matrigel). Whereas both the SNB‐19 and D98^OR^ HeLa parental cells form tumors, four of six hybrid lines did not form tumors, even after periods up to six months, suggesting that each cell type can complement the tumorigenicity of the other. SNB‐19 cells showed high rates of Matrigel invasion at all cell densities examined, whereas D98^OR^ HeLa cells showed lower rates of invasion that were further reduced at high cell density. All six hybrid cell lines displayed a combination of these properties: at low cell density, the hybrids showed high rates of invasion, similar to the SNB‐19 cells, but the invasion rate diminished at higher cell densities, similar to the D98^OR^ HeLa cells. Taken together, these results provide new experimental evidence that several distinct genetic changes are involved in generating the tumorigenic and invasive phenotype of glioblastoma cells. © 1995 Wiley‐Liss, Inc.