Suppression of T-lymphocyte cytotoxicity following exposure to sinusoidally amplitude-modulated fields

✍ Scribed by Daniel B. Lyle; Patricia Schechter; Dr. W. Ross Adey; Robert L. Lundak

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 752 KB
• Volume: 4
• Category: Article
• ISSN: 0197-8462
• DOI: 10.1002/bem.2250040308

✦ Synopsis

Significant inhibition of allogeneic cytotoxicity of the target cell MPC-I 1 by the murine cytotoxic T-lymphocyte line CTLL-I was observed when the 4-h cytotoxicity assay was conducted in the presence of a 450-MHz field sinusoidally amplitude-modulated at 60 Hz. Exposure of the effector cells to the field prior to adding them to the target cells in the cytolytic assay resulted in a similar inhibition, suggesting a direct interaction of the field with the cytolytic T lymphocyte. The inhibition was preferentially expressed during the early allogeneic recognition phase. Fieldexposed cytolytic cells recovered their full cytolytic capacity in 12.5 h. A differential susceptibility was observed with modulation frequencies from 0 to 100 Hz. Peak suppression occurred at 60 Hz modulation, with progressively smaller effects at 40, 16, and 3 Hz. The unmodulated carrier wave did not affect the cytotoxicity. Effects with 80-and 100-Hz modulation were smaller than at 60 Hz. These results demonstrate an inhibitory but recoverable effect by certain amplitude modulations of weak nonionizing radiation upon the cell-mediated cytolytic immune response.