Suppression of Protein Adsorption at Glassy Carbon Electrodes Covalently Modified with Tetraethylene Glycol Diamine
✍ Scribed by Alison J. Downard; Azmi bin Mohamed
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 98 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
Adsorption of high molecular weight species on electrode surfaces can complicate the application of voltammetric techniques to clinical and environmental samples. The performance of tetraethylene glycol diamine as a protective electrode coating has been examined. Glassy carbon electrodes were covalently modi®ed with the diamine via an electrochemically assisted reaction. The voltammetric response of ferrocenemonocarboxylic acid (FCA) at unmodi®ed and modi®ed electrodes in the presence and absence of bovine serum albumin (BSA), lysozyme and ribonuclease (RNase) was used to monitor surface fouling by protein adsorption. Modi®ed electrodes which retained very high sensitivity for FCA oxidation also exhibited excellent resistance to adsorption of all three proteins.