Suppression of penicillin-induced lysis of Staphylococcus aureus by cibacron blue 3G-A
โ Scribed by Motoyuki Sugai; Kaoru Ooku; Tomoko Akiyama; Shingo Inoue; Shigemitsu Iseda; Yoichiro Miyake; Hidekazu Suginaka
- Book ID
- 109317098
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- English
- Weight
- 214 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0378-1097
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๐ SIMILAR VOLUMES
A rapid procedure for the large-scale purification of pig heart nucleoside diphosphate kinase is described. The purification procedure involves extraction of the enzyme, absorption on Cibacron Blue 3G-A Sepharose, elution with ATP, ammonium sulfate precipitation, heat treatment, and rechromatography
Sucrose-phosphate synthase (SPS, E.C. 2.4.1.14) from spinach (Spinacia oleracea L.) was partially purified and the inhibition of the enzyme reaction by 1-deoxynojirimycin and Cibacron blue F3G-A analyzed. Cibacron blue was a high-affinity competitive inhibitor with respect to the substrate UDPglucos