✦ LIBER ✦

Suppression of Lymphocyte Activation by a Protein Released from Isolated Perfused Rat Liver

✍ Scribed by Christopher Pizzo; Donald Lee; Francis V. Chisari

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 1023 KB
Volume: 2
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840020302

No coin nor oath required. For personal study only.

✦ Synopsis

Isolated rat liver perfusates contain a substance which inhibits 3H-thymidine uptake by phytohemagglutinin-stimulated human peripheral blood lymphocytes in a dose-dependent, noncytotoxic fashion. Suppression is not due to interference of lymphocyte-phytohemagglutinin interaction or dilution of the thymidine pool. Complete inhibition of thymidine uptake is achieved with less than 1.0 pg of material per ml (which is a potentially achievable concentration in vivo). The release of this material is directly and quantitatively associated with hepatocellular injury as measured by release of glutamic pyruvate transaminase. The material is a highly basic protein with a molecular weight of approximately 66,000 to 80,000 daltons. It is a product of the hepatocyte rather than of nonparenchymal liver cells. Liver-derived materials, such as the presently described molecule, may play a role in in situ regulation of lymphocyte function during immunologically mediated liver disease.

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