Suppression of human cartilage proteoglycan synthesis by rheumatoid synovial fluid mononuclear cells activated with mycobacterial 60-kd heat-shock protein
✍ Scribed by B. Wilbrink; M. Holewijn; J. W. J. Bijlsma; J. L. A. M. Van Roy; W. Den Otter; W. Van Eden
- Publisher
- John Wiley and Sons
- Year
- 1993
- Tongue
- English
- Weight
- 454 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0004-3591
No coin nor oath required. For personal study only.
✦ Synopsis
Objective. To examine whether T cell reactivity toward heat-shock proteins (HSP) contributes to cartilage destruction in rheumatoid arthritis (RA).
Methods. An in vitro system was used, in which human cartilage explants were cocultured with hsp60activated synovial fluid mononuclear cells (SFMC) from patients with RA, and proteoglycan (PG) synthesis was measured.
Results. The hsp60-activated SFMC suppressed cartilage PG synthesis. This effect was dependent on the production of interleukin-1 (IL-1) and tumor necrosis factor a (TNFa).
Conclusion. Mycobacterial60-kd heat-shock protein can activate rheumatoid SFMC to suppress human