Suppression of a cold-sensitive mutation in ribosomal protein S5 reveals a role for RimJ in ribosome biogenesis
โ Scribed by Biswajoy Roy-Chaudhuri; Narayanaswamy Kirthi; Teresa Kelley; Gloria M. Culver
- Book ID
- 110834928
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 274 KB
- Volume
- 68
- Category
- Article
- ISSN
- 0950-382X
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The nucleotide sequence of the rpsE gene for the ribosomal protein S5 of a mutant of E. coli showing cold-sensitive growth revealed the presence of an insertion sequence, IS1, near the 3'-end of the gene. This mutant grows very slowly even at the "permissive temperature" (30 degrees C). At temperatu
Temperature-sensitive (ts) mutations were isolated within a ribosomal protein gene (rpsL) of Escherichia coli K12. Mutations were mapped by complementation using various transducing phages and plasmids carrying the rpsL gene, having either a normal or a defective promoter for the rpsL operon. One of