Suppressed TGF-β1 expression is correlated with up-regulation of matrix metalloproteinase-13 in keloid regression after flashlamp pulsed-dye laser treatment

✍ Scribed by Yur-Ren Kuo; Wen-Shan Wu; Seng-Feng Jeng; Feng-Sheng Wang; Hui-Chen Huang; Cha-Zon Lin; Kuender D. Yang

• Book ID: 102465045
• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 123 KB
• Volume: 36
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/lsm.20104

✦ Synopsis

Background and Objectives: Flashlamp pulsed-dye lasers (PDLs) has shown effectiveness in the treatment of keloids. In this study, we investigated whether PDL treatments decreased transforming growth factor-b1 (TGF-b1)-induction and up-regulation of matrix metalloproteinase (MMP) expression in keloid regression. Study Design/Materials and Methods: Keloid tissues obtained from 10 patients with intra-lesional or punch biopsies before and 7 days after PDL treatments [fluence per pulse was 10-18 J/cm 2 (mean 14.0 J/cm 2 )]. Immunohistochemical (IHC) staining of TGF-b1 and MMP-1 and MMP-13 expressions in keloid tissue was performed. Western blot analysis of MMP-1 and MMP-13 expressions in extracellular matrix was evaluated. Results: IHC staining indicated that expression of TGF-b1 was significantly reduced in keloid tissues after PDL irradiation. MMP-13 but not MMP-1 expression on IHC staining significantly increased in extracellular matrix of keloid tissues after PDL treatment. Western blot analysis also showed MMP-13 but not MMP-1 significant increased in keloid tissues after PDL treatment. Conclusions: Regression of keloids regressed after PDL treatments are associated with down-regulation of TGF-b1 expression and up-regulation of MMP-13 activity. Lasers