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Supplementation of Substrate Uptake Gene Enhances the Expression of rhIFN-β in High Cell Density Fed-Batch Cultures ofEscherichia coli

✍ Scribed by Anuradha B. Singh, Krishna J. Mukherjee

Book ID: 120899825
Publisher: Humana Press Inc
Year: 2012
Tongue: English
Weight: 606 KB
Volume: 54
Category: Article
ISSN: 1073-6085
DOI: 10.1007/s12033-012-9611-y

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## Abstract Overflow metabolism is an undesirable characteristic of aerobic cultures of __Escherichia coli__. It results from elevated glucose consumption rates that cause a high substrate conversion to acetate, severely affecting cell physiology and bioprocess performance. Such phenomenon typicall