Superoxide anion generation by human neutrophils exposed to monosodium urate. effect of protein adsorption and complement activation
✍ Scribed by Steven Abramson; Sylvia T. Hoffstein; Gerald Weissmann
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 609 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0004-3591
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✦ Synopsis
We studied the capacities of naked and proteincoated monosodium urate (MSU) crystals to stimulate superoxide anion (Ozr) release by human polymorphonuclear leukocytes (PMN). Uncoated MSU stimulated significant Oz2 production by cytochalasin B-treated PMN. Precoating MSU with IgG caused an increase in mean Oz2 production, whereas precoating heated MSU with serum or plasma inhibited Oz2 release. Unheated MSU crystals, which activate complement to a greater extent than heated crystals, also provoked 027 generation, an effect again abrogated by precoating with serum but not with plasma. Coincubation of unheated MSU and plasma resulted in an enhancement of 02T generation. The results of these experiments support the hypothesis that adsorbed proteins modulate the phlogistic potential of MSU and that the surface activation of humoral mediators contributes to the local inflammatory response.
Although it is clear that deposition of crystalline monosodium urate (MSU) crystals in joint tissue can provoke inflammation (l), the cellular events that initiate (and terminate) acute gouty arthritis have not been clarified. Crystal surfaces activate Hageman Factor (2,3), the kinin system (4), and complement components (5-71, each of which might participate in the From the