Supercritical fluid chromatography with Fourier transform infrared and mass spectrometric detection of peracetylated nitrogen derivatives of monosaccharides

✍ Scribed by Elizabeth M. Calvey; John A. G. Roach; Larry T. Taylor; James K. Palmer

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 549 KB
• Volume: 1
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.1220010608

✦ Synopsis

Sugar oximes can be derivatized to yield both the peracetylated oxime and nitrile. The peracetylated oximes do not elute under isothermal (i.e., 200°C) gas chromatography (GC) conditions. When a seven-component derivatized sugar mixture, as determined by GC, was chromatographed under supercritical fluid chromatography (SFC) conditions with a cyanopropyl capillary column, at least 17 peaks were observed. In an effort to identify the additional derivatization products, the eluates were subjected to SFC-FTIR and SFC-MS. The spectral data indicate that cyclic and acyclic oximes as well as nitrile were formed as part of the derivatization method.