Super-resolution imaging in biomedicine

✍ Scribed by Diaspro, Alberto; Zandvoort, Marc A. M. J. van

• Publisher: Taylor & Francis,CRC Press
• Year: 2017
• Tongue: English
• Leaves: 448
• Series: Series in cellular and clinical imaging
• Category: Library

✦ Synopsis

This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market. It covers key techniques such as stimulated emission depletion (STED), structured illumination microscopy (SSIM), photoactivated localization microscopy (PALM), and stochastic optical reconstruction microscopy (STORM). It will be a useful reference for biomedical researchers who want to work with super-resolution imaging, learn the proper technique for their application, and simultaneously obtain a solid footing in other techniques.

✦ Table of Contents

Content: Cover
Half Title
Title Page
Copyright Page
Dedication
Table of Contents
Series Preface
Preface
Editors
Contributors
Section I: Overview of Super-Resolution Technologies
1: In the Realm of Super-Resolved Fluorescence Microscopy 2: Super-Resolution in a Standard Microscope: From Fast Fluorescence Imaging to Molecular Diffusion Laws in Live Cells 3: Photoswitching Fluorophores in Super-Resolution Fluorescence Microscopy
4: Image Analysis for Single-Molecule Localization Microscopy 5: Deconvolution of Nanoscopic Images Section II: The Methods
6: Re-Scan Confocal Microscopy
7: Structured Illumination Microscopy
8: Correlative Nanoscopy: AFM Super-Resolution (STED/STORM)
9: Stochastic Optical Fluctuation Imaging 10: Super-Resolution Two-Photon Excitation Microscopy Utilizing Transmissive Liquid Crystal Devices 11: Fluorescent Nanodiamonds in Biological and Biomedical Imaging and Sensing
Section III: Applications in Biology and Medicine
12: Optical Nanoscopy with SOFI 13: Super-Resolution Fluorescence Microscopy of the Nanoscale Organization of RNAP and DNA in E. coli 14: Correlative Live-Cell and Super-Resolution Microscopy and Its Biological Applications
15: SAX Microscopy and Its Application to Imaging of 3D-Cultured Cells

✦ Subjects

Imaging systems in medicine;Imaging systems in biology;High resolution imaging;HEALTH & FITNESS;Diseases;General;MEDICAL;Clinical Medicine;MEDICAL;Diseases;MEDICAL;Evidence-Based Medicine;MEDICAL;Internal Medicine