✦ LIBER ✦

Sulindac sulfide alters the expression of cyclin proteins in HT-29 colon adenocarcinoma cells

✍ Scribed by Liang Qiao; Steven J. Shiff; Basil Rigas

Publisher: John Wiley and Sons
Year: 1998
Tongue: French
Weight: 152 KB
Volume: 76
Category: Article
ISSN: 0020-7136
DOI: 10.1002/(sici)1097-0215(19980330)76:1<99::aid-ijc16>3.0.co;2-b

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✦ Synopsis

Sulindac sulfide (SS), the active metabolite of the colon cancer chemopreventive compound sulindac, inhibits the proliferation of HT-29 colon cancer cells mainly by inducing cell quiescence. We determined by bivariate flow-cytometric analysis both the DNA and cyclin protein content of individual cells. Thus, we assessed in detail the expression of several cyclins during the cell-cycle phases and demonstrated that SS (i) decreases the expression of cyclins B1 and E and (ii) increases the expression of cyclins D1, D2 and D3, particularly in the G 1 phase of the cell cycle. SS-induced apoptotic cells expressed both E-and D-type cyclins but not cyclin B1. The changes in cyclin expression combined with reduced catalytic activity of cyclin-dependent kinases could explain in molecular terms the anti-proliferative effect of SS on HT-29 colon cancer cells. These changes may contribute to the chemopreventive effect of sulindac.

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