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Sulfotransferases in glycosaminoglycan biosynthesis

✍ Scribed by Marion Kusche-Gullberg; Lena Kjellén

Book ID
113882819
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
179 KB
Volume
13
Category
Article
ISSN
0959-440X

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✍ Kazuyuki Sugahara; Takako Ishii; Ikuo Yamashina 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 748 KB

A method is described for the assay of sulfotransferases, which transfer sulfate from 3'-phosphoadenosine-5'-phosphosulfate (PAPS) to glycosaminoglycan acceptors. Following the sulfation reactions, the [35S]sulfate-labeled products are precipitated and then separated from a sulfate donor ([35S]PAPS)