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Sulfhydryl groups during the s phase: Comparison of cells from G1, plateau-phase G1, and G0

✍ Scribed by J. W. Harris; S. S. Teng

Publisher
John Wiley and Sons
Year
1973
Tongue
English
Weight
434 KB
Volume
81
Category
Article
ISSN
0021-9541

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✦ Synopsis

The non-protein sulfhydryl (NPSH) content of cells moving into S from GI, plateau phase G1, and Go was measured. Chinese hamster ovary (CHO) cells accumulated in G1 by growth into plateau phase contain only onefourth the NPSH concentration of cycling C1 cells or GI cells accumulated by brief growth in isoleucinedeficient medium. Upon dilution of plateau cultures with fresh medium, cellular NPSH content increases rapidly, reaching the same level as that in cycling cells within four hours. This increase is prevented by cycloheximide but not by actinomycin D or hydroxyurea. Neither CHO cells cycling in vitro nor salivary gland Go cells stimulated with isoproterenol in vivo show significant changes in intracellular NPSH concentrations during S phase. This suggests that the concentration of intracellular NPSH (glutathione) remains constant during the cell cycle except when cells are grown to plateau phase in exhausted or deficient medium, in which case normal degradation exceeds synthesis and the gross level falls until fresh medium is provided and synthesis, apparently on preexisting RNA templates, accelerates.

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