Sulfhydryl-dependent thermal aggregation of human gamma globulin

✍ Scribed by Donald A. Gerber

• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: English
• Weight: 559 KB
• Volume: 18
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780180112

✦ Synopsis

Abstract

Hyaluronic acid (4 mg/ml) augmented elevenfold the copper‐catalyzed (7 ~βx~M) thermal (63°C, 2 hours) aggregation of human gamma globulin (2 mg/ ml) in 0.075 M phosphate buffer, pH 7.4. Almost no augmentation of aggregation occurred with hyaluron‐idase‐treated hyaluronate. Hyaluronate‐augmented copper‐catalyzed thermal aggregation was inhibited by L‐histidine, gold thiomalate, N‐ethylmaleimide, p‐chlo‐romercuribenzoic acid, and ethylenediaminetetraacetic acid. Together with previous reports of a decreased blood histidine concentration in rheumatoid arthritis, these studies provide a possible explanation for the affinity of this disease for joints.