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Sulfated Glycopolymer Thin Films—Preparation, Characterization, and Biological Activity

✍ Scribed by Ringo Grombe; Marie F. Gouzy; Manfred F. Maitz; Uwe Freundenberg; Stefan Zschoche; Frank Simon; Tilo Pompe; Claudia Sperling; Carsten Werner

Book ID
102471842
Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
230 KB
Volume
7
Category
Article
ISSN
1616-5187

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✦ Synopsis

Abstract

The impact of heparinoid characteristics on model surfaces obtained from immobilization of sole sulfate groups as well as sulfated glycosides, sulfated cellulose, and definite heparin has been investigated. The obtained layers were physico‐chemically characterized regarding film thickness, chemical composition, wettability, and surface morphology. Antithrombin adsorption, studied by fluorescence labeling, revealed a strong dependence on the presence of glycosidic structures and on the molecular weight of the grafted saccharide. On contact with whole blood, the coatings resulted in a diminished plasmatic and cellular coagulation in vitro, which did not reflect well the antithrombin binding. Therefore, more complex activating pathways are discussed.

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