Sulfanilic acid as surface antigen on mouse lymphoid cells. I. Properties of hapten-labeled cells and effects of antihapten antiserum in vitro and in vivo
✍ Scribed by H. Seiler
- Publisher
- John Wiley and Sons
- Year
- 1972
- Tongue
- English
- Weight
- 656 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0014-2980
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✦ Synopsis
Sulfanilic acid as surface antigen on mouse lymphoid cells lnstitut fur Genetik der Universitat Koln
1. Properties of hapten-labeled cells and effects of antihapten antiserum in vitro and in vivo
The conventional hapten sulfanilic acid (sulf) was coupled covalently t o mouse spleen cells via an azobridge. Survival and functional behavior of the coupled cells were assayed by determining their capacity t o exclude trypan blue, t o secrete antibody and t o produce a secondary immune response in a n adoptive transfer system. Conditions have been found under which the cells were not affected by the coupling procedure but could be specifically lysed or inactivated in vitro and in vivo by anti-sulf antiserum. Inactivation in vivo appeared to require complement, since F(ab')z fragments of anti-sulf antibody did not inactivate coupled cells. The effects of antibody and complement were shown to depend strongly on the amount of hapten coupled to the cells.
The potential of this method is twofold. The hapten can serve as a surface marker, which can be detected by the effect of antiserum in vitro and in vivo. In addition the coupled hapten may be used as an artificial histo- compatibility antigen, which can be manipulated t o a certain extent for experimental analysis of its function.
* Dr. Seiler died in March, 1972, at the age of thirty years.
have been found under which the labeled cells are not affected in their functional behavior in the immune response, but can be completely inactivated in vitro and in vivo by anti-hapten antiserum and complement.
Material and methods
2.1. Antigens
Keyhole limpet hemocyanin (KLII) was purchased from Mann Research Lab., New York; chicken gamma globulin (CGG) was prepared from normal chicken serum (Flow