𝔖 Bobbio Scriptorium
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Suitability of polyethylene glycol for precipitation of monoclonal antibodies— a radiometric study

✍ Scribed by A. Rathinaswamy; J. Chatur; U.H. Nagvekar; N. Ramamoorthy; C.N. Desai

Book ID
103922573
Publisher
Elsevier Science
Year
1991
Weight
198 KB
Volume
42
Category
Article
ISSN
0883-2889

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✦ Synopsis

125I labelled monoclonal antibody (MoAb) to thyroid stimulating hormone (TSH) was used as a tracer for assessing the suitability of polyethylene glycol (PEG) for precipitation of MoAb and MoAb-antigen (AG) complexes. It was found that PEG can be advantageously used under certain conditions favouring insolubility and could thus be a non-specific separation reagent for radioimmunoassays based on MoAbs.

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A simple procedure for the purification of an IgG-type monoclonal antibody by affinity precipitation using Eudragit S-100 is presented. The ligand, a microbial lipase previously used as antigen, was coupled to the polymer at a concentration of 40 mg lipase/g Eudragit. This macroligand was reversibly