๐”– Bobbio Scriptorium
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Suicide selection of murine T helper clones specific for variable regions of the influenza hemagglutinin molecule

โœ Scribed by D. Brian Thomas; John J. Skehel; Kingston H. G. Mills; Christine M. Graham

Book ID
102160813
Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
496 KB
Volume
16
Category
Article
ISSN
0014-2980

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โœฆ Synopsis

A negative selection procedure has been developed to obtain murine T helper clones specific for variable regions of the influenza A hemagglutinin. T cell lines, established from mice primed by intranasal infection with X31 (H3N2) virus, were cross-stimulated with natural variant viruses of known primary sequence (either AITEXASIlI77 or AIENG878169) and proliferating cells eliminated by treatment with the cell cyclespecific drug 5-bromodeoxyuridine. After two suicide cycles, T cell lines were subtype specific and failed to recognize the natural variants. Clones were established by limiting dilution and their specificity was determined against a panel of viruses. Extensive diversity was evident in the reactivity of clones from individual donors, and two major T cell recognition sites were defined in the globular head region of the hemagglutinin molecule.

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