A B-galactosidase gene from Lactobacillus sake coding for lactose hydrolysis was cloned and expressed in Escherichia coli. Chromosomal DNA from L. 'sa& was partially digested with the restriction enzyme SaDAI, and the 3-6 Kb fragment was ligated to the cloning vector pSP72 digested with BarnHI. One
Suicide gene therapy usingE. coliβ-galactosidase
✍ Scribed by David Farquhar; Bih Pan; Mamoru Sakurai; Ajit Ghosh; Craig A. Mullen; Arly J. Nelson
- Publisher
- Springer
- Year
- 2002
- Tongue
- English
- Weight
- 116 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0344-5704
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Following treatment with N-methyl-N'-nitro-N-nitrosoguanidine, 1,257 mutants of Escherichia coli K]2 were isolated on lactose-tetrazolium medium. Of these mutants, 345 were lactose-negative and lacked appreciable fi-galactosidase activity. About half of these enzyme-deficient mutants had lost the wh
## Abstract ## Background Pancreatic cancer remains a rapidly fatal disease. Suicide gene therapy has been shown to be an effective tool for pancreatic tumor cell destruction, but a cell‐specific gene delivery is required to limit host toxicity. The objective of this study was both to design recom