Suicide Gene Therapy: Methods and Protocols

✍ Scribed by Nejat Düzgüneş

Publisher: Springer New York,Humana Press
Year: 2019
Tongue: English
Leaves: 194
Series: Methods in Molecular Biology 1895
Edition: 1st ed.
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

This detailed volume explores the methods used for most of the recent approaches to suicide gene therapy of cancer, which exploits promoters that are specific to cancer cells, thereby ensuring (or greatly increasing the likelihood) that the therapeutic gene is expressed only in cancer cells. The book also contains chapters describing methods to improve the safety of cell therapy and techniques utilizing bone marrow mesenchymal cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Suicide Gene Therapy: Methods and Protocols serves as an ideal guide for researchers expanding upon our knowledge and application of this vital form of cancer therapy.

✦ Table of Contents

Front Matter ....Pages i-xii
Origins of Suicide Gene Therapy (Nejat Düzgüneş)....Pages 1-9
Cancer Suicide Gene Therapy with TK.007 (Jubayer A. Hossain, Kristoffer Riecken, Hrvoje Miletic, Boris Fehse)....Pages 11-26
Osteonectin Promoter-Mediated Suicide Gene Therapy of Prostate Cancer (Wan-Chi Hsiao, Shian-Ying Sung, Leland W. K. Chung, Chia-Ling Hsieh)....Pages 27-42
Suicide Gene Therapy for Oral Squamous Cell Carcinoma (Henrique Faneca, Nejat Düzgüneş, Maria C. Pedroso de Lima)....Pages 43-55
Generation of Suicide Gene-Modified Chimeric Antigen Receptor-Redirected T-Cells for Cancer Immunotherapy (Kentaro Minagawa, Mustafa Al-Obaidi, Antonio Di Stasi)....Pages 57-73
Mesenchymal Stem Cell Exosome-Mediated Prodrug Gene Therapy for Cancer (Cestmir Altaner, Ursula Altanerova)....Pages 75-85
Extracellular Vesicles as Carriers of Suicide mRNA and/or Protein in Cancer Therapy (Erdogan Pekcan Erkan, Nurten Saydam, Clark C. Chen, Okay Saydam)....Pages 87-96
Production of Viral Vectors with Suicide Genes by Utilizing the Intron-Splicing Mechanism of Insect Cells (Haifeng Chen)....Pages 97-109
Resveratrol-Responsive CArG Elements from the Egr-1 Promoter for the Induction of GADD45α to Arrest the G2/M Transition (Qiwen Shi, Deepak Bhatia)....Pages 111-122
Noninvasive Monitoring of Suicide Gene Therapy by Using Multimodal Molecular Imaging (Bryan Holvoet, Cindy Leten, Christophe M. Deroose, Uwe Himmelreich)....Pages 123-134
In Vitro Assay for the Evaluation of Cytotoxic Effects Provided by a Combination of Suicide and Killer Genes in a Bicistronic Vector (Alexey A. Komissarov, Sergey V. Kostrov, Ilya V. Demidyuk)....Pages 135-147
Engineered Thymidine-Active Deoxycytidine Kinase for Bystander Killing of Malignant Cells (Anton Neschadim, Jeffrey A. Medin)....Pages 149-163
Photochemical Internalization Enhanced Nonviral Suicide Gene Therapy (Chung-Ho Sun, Kristian Berg, Henry Hirschberg)....Pages 165-176
Suicide Gene Therapy of Oral Squamous Cell Carcinoma and Cervical Carcinoma In Vitro (Nejat Düzgüneş, Jennifer Cheung, Krystyna Konopka)....Pages 177-184
Back Matter ....Pages 185-190

✦ Subjects

Biomedicine; Gene Therapy; Cancer Research; Molecular Medicine

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